Jhdavis::4x reducing SDS-PAGE loading buffer: Difference between revisions
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New page: 4X SDS-PAGE sample loading buffer From OpenWetWare 10 ml 1X 2.0 ml 1M Tris-HCl, pH 6.8 50 mM 0.8 g SDS 2% 4.0 ml 100% glycerol 10% 0.4 ml 14.7 M -mercaptoethanol 1% 1.0 ... |
New page: 4X SDS-PAGE sample loading buffer From OpenWetWare 10 ml 1X 2.0 ml 1M Tris-HCl, pH 6.8 50 mM 0.8 g SDS 2% 4.0 ml 100% glycerol 10% 0.4 ml 14.7 M -mercaptoethanol 1% 1.0 ... |
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Latest revision as of 18:27, 6 May 2009
4X SDS-PAGE sample loading buffer From OpenWetWare
10 ml 1X
2.0 ml 1M Tris-HCl, pH 6.8 50 mM
0.8 g SDS 2%
4.0 ml 100% glycerol 10%
0.4 ml 14.7 M -mercaptoethanol 1%
1.0 ml 0.5 M EDTA 12.5 mM
8.0 mg bromophenol blue 0.02%
2.6 ml H2O
Use at 1X for loading samples onto SDS-polyacrylamide gels.
