Yu:TEV Cleavage Buffer: Difference between revisions
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|+'''Solution recipe for | |+'''Solution recipe for 500 mL TEV Cleavage Buffer.''' | ||
|width="66%" valign="top"|1. Measure 100 mL millipore H<sub>2</sub>O into a 500mL beaker | |width="66%" valign="top"|1. Measure 100 mL millipore H<sub>2</sub>O into a 500mL beaker | ||
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|5. Bring volume to 500L with millipore H<sub>2</sub>O in a graduated cylinder | |5. Bring volume to 500L with millipore H<sub>2</sub>O in a graduated cylinder | ||
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|6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB | |6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB | ||
Latest revision as of 18:58, 24 December 2009
| 1. Measure 100 mL millipore H2O into a 500mL beaker | |
| 2. Add 12.5 mL of 1M TRIS-HCl, pH 8.0 | 25 mM TRIS-HCl |
| 3. Add 15 mL of 5M NaCl | 150 mM NaCl |
| 4. Add 500 μL of 0.5M EDTA | 0.5 mM EDTA |
| 5. Add 5 mL 10% NP-40 | 0.1% NP-40 |
| 5. Bring volume to 500L with millipore H2O in a graduated cylinder | |
| 6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB | 1 mM DTT |
