Yu:Dialysis: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
dialysis |
m typo |
||
| Line 3: | Line 3: | ||
{| border="0" cellpadding="5" | {| border="0" cellpadding="5" | ||
|+'''SpectraPor Membrane Dialysis''' | |+'''SpectraPor Membrane Dialysis''' | ||
|valign="top"|1. Cut an appropriate length of dialysis tubing. You will need | |valign="top"|1. Cut an appropriate length of dialysis tubing. You will need 5cm for tying, plus 5cm tubing per 1mL. | ||
|- | |- | ||
|2. Soak in a large volume of distilled H<sub>2</sub>O for 30 minutes | |2. Soak in a large volume of distilled H<sub>2</sub>O for 30 minutes | ||
Revision as of 14:06, 18 May 2010
| 1. Cut an appropriate length of dialysis tubing. You will need 5cm for tying, plus 5cm tubing per 1mL. |
| 2. Soak in a large volume of distilled H2O for 30 minutes |
| 3. Soak in a large volume of 2% Sodium Bicarbonate (w/v), 1mM EDTA at 60°C for 30 minutes |
| 4. Rinse thoroughly in a large volume of distilled H2O. |
| 5. Carefully tie off one end of the tubing with a double-knot. |
| 6. Add solution to be dialyzed by pipetting. |
| 7. Clamp off the open end with a plastic floating clamp. |
| 8. Suspend in the dialysis solution in a large beaker or graduated cylinder, depending on the length of the dialysis tubing. |
| 9. Change dialysis solution as needed. |
| 10. When dialysis is complete, carefully pinch off the tubing under the clamp. Remove the clamp carefully - note that the tubing will break where it was clamped if you are not supporting the tubing. |
| 11. Carefully decant the dialyzed solution by pipetting or by pouring. |
