BME103:T930 Group 2 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Ryan Sullivan Research Development Scientist Miriam Y Acosta PCR Machine Engineer Ryan Keeney PCR Machine Engineer Juliana Ramos Experimental Protocol Planner Aaron Cornejo Experimental Protocol Planner LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features Instructions Protocols Materials Supplied in the Kit Amount Open PCR Machine 1 10 μM Forward Primer 16.0 μL 10 μM Reverse Primer 16.0 μL GoTaq Master Mix 800.0 μL dH2O 764.8 μL Tubes 16 Fluorimeter 1 Teflon Glass Slides 16 Alan Wrench 1 Operations Manual 1 Supplied by User Amount Screwdriver 1 Template DNA (20ng) 3.2 μL Micro-Pipetter 1 Camera Phone 1 PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers Sample A Sickle Cell Anemia rs35685286 [Homo sapiens] GGATGAAGTTGGTGGT--GAGGCCCTGG[A/G]CAGGTTGGTA--TCAAGGTTACAAGAC Chromosome 11- single nucleotide variation http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=35685286 Sample B Sickle Cell Anemia rs34430836 [Homo sapiens] AGGTGCTAGGTGCCTT--TAGTGATGGC[C/G]TGGCTCACCT--GGACAACCTCAAGGG Chromosome 11- single nucleotide variation http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=34430836 Primer Design Sample A rs35685286 [Homo sapiens] Primer--CTCCGGGACCTGTCCAACCAT Reverse Primer-- GAGGCCCTGGACAGGTTGGTA Sample B rs34430836 [Homo sapiens] Primer—ATCACTACCGGACCGAGTGGA Reverse Primer—TAGTGATGGCCTGGCTCACCT Illustration