James P. McDonald Week 3: Difference between revisions

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#'''''Glutamate''''': "Major fast excitatory neurotransmitter in the mammalian central nervous system." [[http://www.biology-online.org/dictionary/Glutamate]]
#'''''Glutamate''''': "Major fast excitatory neurotransmitter in the mammalian central nervous system." [[http://www.biology-online.org/dictionary/Glutamate]]
#'''''Glutamine''''': "A crystalline amino acid occurring in proteins; important in protein metabolism. One of the 20 amino acids that are commonly found in proteins." [[http://www.biology-online.org/dictionary/Glutamine]]
#'''''Glutamine''''': "A crystalline amino acid occurring in proteins; important in protein metabolism. One of the 20 amino acids that are commonly found in proteins." [[http://www.biology-online.org/dictionary/Glutamine]]
#'''''GAP1''''': "General amino acid permease, a gene found in <i>Saccharomyces cerevisiae</i>." [[http://amigo.geneontology.org/cgi-bin/amigo/gp-details.cgi?gp=SGD:S000001747&session_id=9053amigo1359594666]]
#'''''GAP1''''': "General amino acid permease, a gene found in ''Saccharomyces cerevisiae''." [[http://amigo.geneontology.org/cgi-bin/amigo/gp-details.cgi?gp=SGD:S000001747&session_id=9053amigo1359594666]]
#'''''PUT4''''': "Proline permease, a gene found in <i>Saccharomyces cerevisiae</i>." [[http://amigo.geneontology.org/cgi-bin/amigo/gp-details.cgi?gp=SGD:S000005875&session_id=9053amigo1359594666]]
#'''''PUT4''''': "Proline permease, a gene found in ''Saccharomyces cerevisiae''." [[http://amigo.geneontology.org/cgi-bin/amigo/gp-details.cgi?gp=SGD:S000005875&session_id=9053amigo1359594666]]


==Outline==
==Outline==
===Introduction===
*''Saccharomyces cerevisiae'' was grown in various ammonia concentrations and the effects on the growth was observed.
**A single dilution rate was using with a range of different ammonia concentrations.
**The ammonia concentrations were varied to observe its effects on gene expression and enzyme activities.
*The main result of the study was that nitrogen metabolism is dependent on ammonia concentration, not its flux.
===Methods===
====Physiological Parameters====
====Northern Analyses====
====Enzyme Activities====


{{James P. McDonald}}
{{James P. McDonald}}


[[Category:BIOL398-03/S13]]
[[Category:BIOL398-03/S13]]

Revision as of 01:55, 31 January 2013

Biological Terms

  1. Permease: "General term for a membrane protein that increases the permeability of the plasma membrane to a particular molecule, by a process not requiring metabolic energy." [[1]]
  2. Isomerase: "An enzyme that converts molecules into their positional isomers." [[2]]
  3. Oligonucleotides: "Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesised to match a region where a mutation is known to occur, and then used as a probe (oligonucleotide probes)." [[3]]
  4. Dehydrogenase: "Enzyme that oxidizes a substrate by transferring hydrogen to an acceptor that is either NAD/NADP or a flavin enzyme. An enzyme that is used to remove hydrogen from its substrate, which is used in the cytochrome (hydrogen carrier) system in respiration to produce a net gain of ATP." [[4]]
  5. Synthetase: "Enzymes of class 6 in the e classification, catalyse synthesis of molecules, their activity being coupled to the breakdown of a nucleotide triphosphate." [[5]]
  6. Biosynthetic: "Relating to or produced by biosynthesis." [[6]]
  7. Glutamate: "Major fast excitatory neurotransmitter in the mammalian central nervous system." [[7]]
  8. Glutamine: "A crystalline amino acid occurring in proteins; important in protein metabolism. One of the 20 amino acids that are commonly found in proteins." [[8]]
  9. GAP1: "General amino acid permease, a gene found in Saccharomyces cerevisiae." [[9]]
  10. PUT4: "Proline permease, a gene found in Saccharomyces cerevisiae." [[10]]

Outline

Introduction

  • Saccharomyces cerevisiae was grown in various ammonia concentrations and the effects on the growth was observed.
    • A single dilution rate was using with a range of different ammonia concentrations.
    • The ammonia concentrations were varied to observe its effects on gene expression and enzyme activities.
  • The main result of the study was that nitrogen metabolism is dependent on ammonia concentration, not its flux.

Methods

Physiological Parameters

Northern Analyses

Enzyme Activities

Journal Entries and Assignments

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