BME494s2013 Project Team1

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Overview & Purpose Text describing the image Sarah Background Text describing the image Sean Design: Our genetic circuit Julia OUR GENE SWITCH: Building: Assembly Scheme Emily Testing: Modeling and GFP Imaging A LAC SWITCH MODEL We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated. AN INTERACTIVE MODEL We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system. COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions. Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model. Sarah and Julia Human Practices Danger of Chemicals in Farmlands Our Team Your Name My name is Emily Byrne, and I am a student majoring in biomedical engineering. I am taking BME 494 because ###. An interesting fact about me is that ###. Sarah K. Halls My name is Sarah K. Halls, and I am a student majoring in Biomedical Engineering. I am taking BME 494 because I enjoy cell and tissue Engineering work and hope to start my career in this field of study. An interesting fact about me is that I did an internship at Harvard University working on cell patterning. Sean Hector My name is Edgil Hector (Sean), and I am a student majoring in biomedical engineering. I am taking BME 494 because the subject is relevant to my interests, and the class counts as a required technical elective. An interesting fact about me is that I am the most indecisive human being on the planet. Julia Smith My name is Julia Smith, and I am a senior majoring in Biomedical Engineering. I am taking BME 494 because I am extremely interested in synthetic biology. An interesting fact about me is that in addition to my nerdy side and love of accademic learning, I train reining horses. Works Cited [1] Full reference. [2] Full reference. [3] Full reference.