Strain cells: Difference between revisions

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==Concepts and Comments==
==Concepts and Comments==
The question here is "Can we use a strainer to separate cells from medium?" The idea being to eliminate a centrifugation step when exchanging to sulfur free medium. Ideas for strainers are a fine screen or mesh or even a microporous filter, such as a membrane.
Another possibility to test for separating cells from medium is settling. If circulation is turned off, will cells settle enough to where the medium can be carefully drained off?
==Materials==
==Materials==
==Links==
==Links==

Latest revision as of 02:36, 1 August 2005

Concepts and Comments

The question here is "Can we use a strainer to separate cells from medium?" The idea being to eliminate a centrifugation step when exchanging to sulfur free medium. Ideas for strainers are a fine screen or mesh or even a microporous filter, such as a membrane.

Another possibility to test for separating cells from medium is settling. If circulation is turned off, will cells settle enough to where the medium can be carefully drained off?

Materials