IGEM:MIT/2007/Notebook/2007-7-9: Difference between revisions
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===Nanodrop of Purified pSB3k3 from Mini-Prep of DB3.1 LQ=== | ===Nanodrop of Purified pSB3k3 from Mini-Prep of DB3.1 LQ=== | ||
1. 38.9 ng/µl | :1. 38.9 ng/µl | ||
2. 38.7 ng/µl | :2. 38.7 ng/µl | ||
3. 33.7 ng/µl | :3. 33.7 ng/µl | ||
4. 33.8 ng/µl | :4. 33.8 ng/µl | ||
===Digestion of Purified pSB3k3 from this morning=== | ===Digestion of Purified pSB3k3 from this morning=== | ||
Revision as of 20:37, 9 July 2007
Nanodrop of Purified pSB3k3 from Mini-Prep of DB3.1 LQ
- 1. 38.9 ng/µl
- 2. 38.7 ng/µl
- 3. 33.7 ng/µl
- 4. 33.8 ng/µl
Digestion of Purified pSB3k3 from this morning
Digestion 1:
- 25.6 µl pSB3k3 (38.9 ng/µl)
- 0.5 µl BSA
- 5.0 µl NEB3 Buffer
- 1.0 µl EcoRI
- 1.0 µl PstI
- 16.9 µl H2O
Digestion 2:
- 25.8 µl pSB3k3 (38.7 ng/µl)
- 0.5 µl BSA
- 5.0 µl NEB3 Buffer
- 1.0 µl EcoRI
- 1.0 µl PstI
- 16.7 µl H2O
- Placed in 37C room at 4:30pm
Liquid Culture of DB3.1
Took two colonies from each plate (1A, 1B, 2A, 2B, 3A, 3B)
Poured Plates
- 16 LB+Amp (180µl in 450ml LB)
- 28 LB+Kan (475µl in 475ml LB)
Purification of pSB3k3, F2620 and B0034 Digestion
pSB3k3: 3.5 ng/µl F2620: 17.7 ng/µl B0034: 19.9 ng/µl
- Because B0034 is under 200bp, we had to use a different kit (Qiagen Nucleotide Removal Kit)
- For pSB3k3 and F2620, we used the Qiagen PCR Purification Kit
