IGEM:MIT/2007/Notebook/2007-7-10: Difference between revisions

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XL-1 in Kan (300µl) - 800
XL-1 in Kan (300µl) - 800
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===Innoculated 4 Liquid Cultures of Transformation from XL-1 in Kan (20µl) Plate===
*Used 5ml of LB+Kan
*Placed in 37C room at around 12pm
===Sent out F2620, B0034, E1010 for Sequencing===
*Used samples #2 because those were also used for the digestion/ligation/3A
*Form 7942
*Used 3µl of each miniprepped DNA sample, 1µl of VF2/VR, and 8µl H2O (12µl total)

Revision as of 19:54, 10 July 2007

Checked Transformation Plates

DH5a in Amp (100µl) - 14 colonies
DH5a in Amp (300µl) - 60
DH5a in Kan (100µl) - 4
DH5a in Kan (300µl) - 10

XL-1 in Amp (20µl) - 720 colonies
XL-1 in Amp (200µl) - 4800
XL-1 in Kan (20µl) - 220
XL-1 in Kan (300µl) - 800

Innoculated 4 Liquid Cultures of Transformation from XL-1 in Kan (20µl) Plate

  • Used 5ml of LB+Kan
  • Placed in 37C room at around 12pm

Sent out F2620, B0034, E1010 for Sequencing

  • Used samples #2 because those were also used for the digestion/ligation/3A
  • Form 7942
  • Used 3µl of each miniprepped DNA sample, 1µl of VF2/VR, and 8µl H2O (12µl total)
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