Skatebro:Sequencing Prep: Difference between revisions
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New page: ==Steps== #Reconstitute primer of X nMoles by adding 40*X uL of water, making 25 uM solution #Centrifuge for 15 seconds. #Add 180ng of template DNA #Add 3.2 pMoles of primer (0.128 uL fr... |
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Revision as of 04:12, 1 February 2008
Steps
- Reconstitute primer of X nMoles by adding 40*X uL of water, making 25 uM solution
- Centrifuge for 15 seconds.
- Add 180ng of template DNA
- Add 3.2 pMoles of primer (0.128 uL from 25 uM working solution)
