Skatebro:Project08: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 18: | Line 18: | ||
==Tools== | ==Tools== | ||
*[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed pubmed] | *[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed pubmed] | ||
*[http://www.neb.com/nebecomm/default.asp New England BioLabs] | |||
*[http://www.ncbi.nlm.nih.gov/blast/Blast.cgi BLAST] | *[http://www.ncbi.nlm.nih.gov/blast/Blast.cgi BLAST] | ||
*[http://parts.mit.edu/registry/index.php/Main_Page Registry Main Page] | *[http://parts.mit.edu/registry/index.php/Main_Page Registry Main Page] | ||
| Line 29: | Line 30: | ||
*[http://tools.neb.com/NEBcutter2/index.php NEBcutter RE analyzer] | *[http://tools.neb.com/NEBcutter2/index.php NEBcutter RE analyzer] | ||
*[http://www.thelabrat.com/restriction/index.shtml RE compatability] | *[http://www.thelabrat.com/restriction/index.shtml RE compatability] | ||
*[http://ecocyc.org/ ecocyc e. coli metabolic pathways] | *[http://ecocyc.org/ ecocyc e. coli metabolic pathways] | ||
*[http://cgsc.biology.yale.edu/index.php CGSC coli genetic stock center] | *[http://cgsc.biology.yale.edu/index.php CGSC coli genetic stock center] | ||
| Line 35: | Line 35: | ||
*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp recommended RE cleavage overhangs] | *[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp recommended RE cleavage overhangs] | ||
*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_activity_restriction_enzymes.asp? NEB buffers] | *[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_activity_restriction_enzymes.asp? NEB buffers] | ||
*[http://www.neb.com/nebecomm/products/productF-530.asp phusion dna polymerase manual] | |||
Revision as of 00:41, 8 February 2008
Protocols
- Skatebro:Pouring_Plates
- Skatebro:Primer_Design
- Skatebro:PCR
- Skatebro:Digestion
- Skatebro:Ligation
- Skatebro:Transformation
- Skatebro:Electrophoresis_Gels
- Skatebro:Sequencing_Prep
Notes
- Papers: Skatebro:Notes_Papers
- Presentations: Skatebro:Notes_Presentations
- Other: Skatebro:Notes_Other
Tools
- pubmed
- New England BioLabs
- BLAST
- Registry Main Page
- random DNA sequence generator
- reverse translate protein -> dna
- design a codon optimized gene
- Non-functional DNA sequences
- stock dilution calculator
- IDT oligoanalyzer
- the genetic code
- NEBcutter RE analyzer
- RE compatability
- ecocyc e. coli metabolic pathways
- CGSC coli genetic stock center
- Vera MIT journal reserve search
- recommended RE cleavage overhangs
- NEB buffers
- phusion dna polymerase manual
