SEED/2008/Day 5: Difference between revisions
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== Morning == | == Morning == | ||
* | * gel purification | ||
* | * Digest PCRs with XbaI/PstI | ||
== Afternoon == | == Afternoon == | ||
* | * "PCR cleanup" the insert digests | ||
== Instructor Preparation == | == Instructor Preparation == | ||
Revision as of 23:22, 7 March 2008
Morning
- gel purification
- Digest PCRs with XbaI/PstI
Afternoon
- "PCR cleanup" the insert digests
Instructor Preparation
- oligos with different promoters from this set registry:Part:BBa_J23101 pre-cut with EcoRI/SpeI
- T4 PNK top/bottom oligos
- mix and anneal
Post-prep
- Remove plates from incubator
