Love:Protocols: Difference between revisions
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*[[Love:Extract unreacted monomer|Extract unreacted monomer]] | *[[Love:Extract unreacted monomer|Extract unreacted monomer]] | ||
*[[Love:Plasma cleaning|Plasma cleaning]] | *[[Love:Plasma cleaning|Plasma cleaning]] | ||
*[[Love:Preparing poly-lysine slides|Preparing poly-lysine slides]] | |||
===Microengraving (μEn)=== | ===Microengraving (μEn)=== | ||
Revision as of 15:00, 24 April 2008
Common Buffers and Blocking Solutions
Buffers for Processing Protein Microarrays
- Blocking solution stock for Antibody Arrays- PBS/3%BSA
- Washing solution stock - PBS/1% Tween 20 (PBST 1%)
Tissue Culture Media
- General DMEM media for hybridomas (DMEM-10)
- Cell fusion-Myeloma partner cells
- Cloning media for single cells (DMEM-20+HCF)
Mammalian Cell Culture
General Procedures
Hybridomas
others
Immunization
Probes
