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Lab note books

Please follow this format for your entries

• Date(year/month/day)-Hard copy lab book reference (Initials lab book #-page)
• Title of experiment with brief description
• Experimental procedures: Refer to appropriate protocol and note any changes in them
• Results: Post pictures with caption and appropriate identification of lanes on gels
  • State what are your finding and any comments

2008/06/30 - AP06-39

Expression assay on positives clones

Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression

Experimental procedures

• Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL) to OD~0.4
• Induced with 0.1 mM IPTG (100 µL of 100 mM stock)
• Incubated in shaker B at 37°C O/N
• Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else)

Results

SDS-PAGE 15% 200V 45 min

Lane	Sample	Volume
1	Marker PS	8 µL
2	Sample	Vol µL
3	Sample	Vol µL
4	Sample	Vol µL
5	Sample	Vol µL
6	Sample	Vol µL
7	Sample	Vol µL
8	Sample	Vol µL
9	Sample	Vol µL
10	Sample	Vol µL
11	Sample	Vol µL
12	Sample	Vol µL
13	Sample	Vol µL
14	Sample	Vol µL
15	Sample	Vol µL