IGEM:Peking University/2008/Experiment/PKU 080514 G1 1: Difference between revisions
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::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul''' | ::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul''' | ||
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[[ | [[Image:PKU_080514_ADH-PCR-check.tif|400px]] | ||
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Revision as of 16:59, 3 July 2008
PKU_080514_G1_1
- PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
| Volume (μL) | ||
| pGREG505/(TEF-lacI-adhlacS-GFP) | 1 | |
| easytaq mixture | 25 | *3 |
| F-Primer | 0.5 | |
| R-Primer | 0.5 | |
| ddH2O | 23 |
- conditions
| Temperature(°C) | Time Length(0:00:00) | |
| 94 | 05:00 | |
| 94 | 00:30 | 30 cycles |
| 50.1,52.2,55.1,58.3,62.2 | 01:00 | 30 cycles |
| 72 | 01:30 | 30 cycles |
| 72 | 07:00 | |
| Alarm | 5 times | |
| Hold | 4°C |
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