Annexin-V: Difference between revisions
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New page: * Conjugated to other fluorochromes (PE, FITC) * Stains cells in '''early stage of apoptosis''' ** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane... |
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* Stains cells in '''early stage of apoptosis''' | * Stains cells in '''early stage of apoptosis''' | ||
** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis | ** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis | ||
* Must be used in conjunction with vital dye (e.g. [[Propidium iodide|PI]], [[7-AAD]]) to distinguish dead cells (Annexin-V +/7-AAD +) from apoptotic cells (Annexin-V +/7-AAD -) | |||
Revision as of 21:19, 6 July 2008
- Conjugated to other fluorochromes (PE, FITC)
- Stains cells in early stage of apoptosis
- Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
- Must be used in conjunction with vital dye (e.g. PI, 7-AAD) to distinguish dead cells (Annexin-V +/7-AAD +) from apoptotic cells (Annexin-V +/7-AAD -)
