IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week8/Chemical and Light: Difference between revisions
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Nothing looks hopeful, so we'll wait until sequences come back. | Nothing looks hopeful, so we'll wait until sequences come back. | ||
Good luck! | |||
Revision as of 19:32, 11 August 2008
MIT lacZ parts RE digests 08/11
|
custom made for Meng Xiao | |
|---|---|---|
| Park Lane | Table of Contents | |
| 1 | 1 kb ladder | |
| 2 | P109 MIT cut ES (cut out insert 3093; vector 2079) | |
| 3 | P110 MIT cut XP (insert 534; vector 3953) | |
| 4 | P111 MIT cut XP (insert 534; cut out vector 3755) | |
| 5 | P113 MIT cut XP (insert 534; vector 3973) | |
| 6 | P112 MIT cut XP (cut out insert 534; vector 3722) | |
P108+45 PCR, P1+mtrB test digest 8/11
|
1.2% E-gel visualized using EtBr/UV | |
|---|---|---|
| Lane | Contents (approx. expected band size) | |
| 1-4 | P108+45 putative colony PCR #12-15 (2.3kb) | |
| 2 | P1+mtrB not BB test digest (ES) #82 (2.2+2.75kb) | |
| 3 | P1+mtrB not BB test digest (ES) #87 (2.2+2.75kb) | |
| 4 | P1+mtrB not BB test digest (ES) #95 (2.2+2.75kb) | |
| 5-12 | 1kb ladder | |
Nothing looks hopeful, so we'll wait until sequences come back.
Good luck!
