Template:Imperial/Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m New page: {| cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="left" width=50% |width="100%"| <font size=4px><font color=white>'''1. Transformation Protocols f... |
No edit summary |
||
| Line 1: | Line 1: | ||
<html><table cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="left" width=50%> | |||
<tr><td width="100%"> <font size=4px><font color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''</td></tr> | |||
<tr><td style="background:#FFFFFF"> | |||
</html>*[[/Transformation_protocol_2 | Protocol 1]]<html> | |||
*[[/Transformation_protocol_2 | Protocol 1]] | </html>*[[/Transformation_protocol_4 | Protocol 2]]<html></td></tr> | ||
*[[/Transformation_protocol_4 | Protocol 2]] | <tr><td style="background:#66aadd"><font size=4px><font color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''</td></tr> | ||
<tr><td style="background:#FFFFFF"> | |||
</html>*Protocol<html></td></tr> | |||
<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''</td></tr> | |||
<tr><td style="background:#FFFFFF"> | |||
*Protocol | </html>*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]<html></td></tr> | ||
<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''4. Motility Analysis'''</td></tr> | |||
<tr><td style="background:#FFFFFF"> | |||
</html>*[[/Protocols#Motility_Experiments | Motility Assays]]<html></td></tr> | |||
<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''5. PCR'''</td></tr> | |||
*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]] | <tr><td style="background:#FFFFFF"> | ||
</html>*[[/PCR| PCR]]<html> | |||
</html>*[[/Colony_PCR| Single Colony PCR]]<html></td></tr> | |||
<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''6. Calibration Curves'''</td></tr> | |||
<tr><td style="background:#FFFFFF"> | |||
*[[/Protocols#Motility_Experiments | Motility Assays]] | </html>*[[/Calibration-Curves| Calibration Curves]]<html> | ||
</html>*[[/Growth| O.D.<sub>600</sub> vs cell number]]<html> | |||
</html>*[[/Fluorescence| Fluorescence vs GFPmut3b]]<html></td></tr> | |||
<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''7. Promoter-RBS Testing Protocols'''</td></tr> | |||
<tr><td style="background:#FFFFFF"> | |||
*[[/PCR| PCR]] | </html>*[[/Constitutive | Constitutive Promoters and RBS]]<html> | ||
*[[/Colony_PCR| Single Colony PCR]] | </html>*[[/Inducible | pHyperspank Promoter and RBS]]<html> | ||
</html>*[[/Light_Inducible | Light Inducible Promoters and RBS]]<html></td></tr> | |||
<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''8. Biomaterial Protocols'''</td></tr> | |||
<tr><td style="background:#FFFFFF"> | |||
</html>*Protocol<html></td></tr> | |||
*[[/Calibration-Curves| Calibration Curves ]] | </table></html> | ||
*[[/Growth| O.D.<sub>600</sub> vs cell number]] | |||
*[[/Fluorescence| Fluorescence vs GFPmut3b]] | |||
*[[/Constitutive | Constitutive Promoters and RBS]] | |||
*[[/Inducible | pHyperspank Promoter and RBS]] | |||
*[[/Light_Inducible | Light Inducible Promoters and RBS]] | |||
*Protocol | |||
Revision as of 14:00, 18 September 2008
<html>
| 1. Transformation Protocols for integration vectors in Bacillus subtilis |
|
</html>* Protocol 1<html> </html>* Protocol 2<html> |
| 2. Transformation Protocol for linear DNA in Bacillus subtilis |
| </html>*Protocol<html> |
| 3. Transformation Protocol E.coli |
| </html>* Preparation of XL1-blue electrocompetent cells<html> |
| 4. Motility Analysis |
| </html>* Motility Assays<html> |
| 5. PCR |
|
</html>* PCR<html> </html>* Single Colony PCR<html> |
| 6. Calibration Curves |
|
</html>* Calibration Curves<html> </html>* O.D.600 vs cell number<html> </html>* Fluorescence vs GFPmut3b<html> |
| 7. Promoter-RBS Testing Protocols |
|
</html>* Constitutive Promoters and RBS<html> </html>* pHyperspank Promoter and RBS<html> </html>* Light Inducible Promoters and RBS<html> |
| 8. Biomaterial Protocols |
| </html>*Protocol<html> |
</html>
