IGEM:IMPERIAL/2008/New/BioBricks: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
mNo edit summary
mNo edit summary
Line 46: Line 46:
|- style=background:mediumslateblue
|- style=background:mediumslateblue
| K143005 || 5' EpsE Integration Sequence || PCR Verified
| K143005 || 5' EpsE Integration Sequence || PCR Verified
|- style=background:mediumslateblue
|- style=background:#cc4400
| K143006 || 3' EpsE Integration Sequence || PCR Verified
| K143006 || 3' EpsE Integration Sequence || Sent for Sequencing
|- style=background:yellow
|- style=background:yellow
| K143008 || 5' PyrD Integration Sequence || Pending
| K143008 || 5' PyrD Integration Sequence || Pending
|- style=background:mediumslateblue
|- style=background:mediumslateblue
| K143009 || 3' PyrD Integration Sequence || PCR verified
| K143009 || 3' PyrD Integration Sequence || PCR verified
|- style=background:mediumslateblue
|- style=background:#cc4400
| K143031 || Aad9 Spectinomycin Resistance Gene || PCR Verified
| K143031 || Aad9 Spectinomycin Resistance Gene || Sent for Sequencing
|- style=background:silver
|- style=background:silver
| K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Sequenced
| K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Sequenced

Revision as of 12:45, 9 October 2008

<html> <style type="text/css"> .firstHeading {display: none;} </style> </html> <html> <style type="text/css">

   table.calendar          { margin:0; padding:2px; }

table.calendar td { margin:0; padding:1px; vertical-align:top; } table.month .heading td { padding:1px; background-color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } table.month .dow td { text-align:center; font-size:110%; } table.month td.today { background-color:#3366FF } table.month td {

   border:2px;
   margin:0;
   padding:0pt 1.5pt;
   font-size:8pt;
   text-align:right;
   background-color:#FFFFFF;
   }
  1. bodyContent table.month a { background:none; padding:0 }

.day-active { font-weight:bold; } .day-empty { color:black; } </style> </html>

<html><script language="JavaScript">

var timeout = 250; var closetimer = 0; var ddmenuitem = 0;

// open hidden layer function mopen(id) { // cancel close timer mcancelclosetime(); // close old layer if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; // get new layer and show it ddmenuitem = document.getElementById(id); ddmenuitem.style.visibility = 'visible'; } // close showed layer function mclose() { if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; } // go close timer function mclosetime() { closetimer = window.setTimeout(mclose, timeout); } // cancel close timer function mcancelclosetime() { if(closetimer) { window.clearTimeout(closetimer); closetimer = null; } } // close layer when click-out //document.onclick = mclose; </script>




</html>
<html><style type="text/css">

div.Section { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; background-image: url(http://openwetware.org/images/a/a0/Background.PNG); background-size: 100%; background-origin: content; }

/* Text (paragraphs) */ div.Section p { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:justify; margin-top:0px; margin-left:30px; margin-right:30px; }

/* Headings */ div.Section h1 { font:22pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:left; color:#3366FF; font-weight:bold; }

/* Subheadings */ div.Section h2 { font:18pt Calibri, Verdana, Arial, Geneva, sans-serif; color:#3366FF; margin-left:5px; font-weight:bold; }

/* Subsubheadings */ div.Section h3 { font:22pt Calibri, Verdana, Arial, sans-serif; color:#E5EBFF; margin-left:10px; font-weight:bold; }

/* Subsubsubheadings */ div.Section h4 { font:22pt Calibri, Verdana, Arial, sans-serif; color:#2B48B3; margin-left:10px; font-weight:bold; }

/* Subsubsubsubheadings */ div.Section h5 { font:12pt Calibri, Verdana, Arial, sans-serif; color:#3366FF; margin-left:20px; }

/* References */ div.Section h6 { font:12pt Calibri, Verdana, Arial, sans-serif; font-weight:bold; font-style:italic; color:#3366FF; margin-left:25px; }

/* Hyperlinks */ div.Section a { }

div.Section a:hover { }

/* Tables */ div.Section td { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:justify; vertical-align:top; padding:2px 4px 2px 4px; }

/* Lists */ div.Section li { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:left; margin-top:0px; margin-left:30px; margin-right:0px; }

/* TOC stuff */ table.toc { margin-left:10px; }

table.toc li { font: 11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align: justify; margin-top: 0px; margin-left:2px; margin-right:2px; }

/* [edit] links */ span.editsection { color:#BBBBBB; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; }

span.editsection a { color:#BBBBBB; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; }

span.editsection a:hover { color:#3366FF; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; }

/* Drop-down Menu */

  1. sddm {

margin: 0; padding: 0; z-index: 30 margin: 0; padding: 0; float: center; font: bold 12pt Calibri, Verdana, Arial, Geneva, sans-serif; border: 0px; list-style: none; }

  1. sddm a {

display: block; margin: 0px 0px 0px 0px; padding: 0 0 12px 0; color: #FFFFFF; text-align: center; text-decoration: none; }

  1. sddm a:hover {

border: 0px }

  1. sddm div {

position: absolute; visibility: hidden; margin: 0; padding: 0; background: #66aadd; border: 1px solid #66aadd } #sddm div a { position: relative; left: 0; display: block; margin: 0; padding: 5px 10px; width: auto; white-space: nowrap; text-align: left; text-decoration: none; background: #FFFFFF; color: #2875DE; font: 11pt Calibri, Verdana, Arial, Geneva, sans-serif } #sddm div a:hover { background: #66aadd; color: #FFFFFF } </style></html>


BioBricks & Characterisation
Device 1 
Device 2 

Parts Submitted to the Registry

Production Status

This table shows our progress with each part:


Key
Submitted
Sequenced
Midiprepped
Fully verified
Partially Verified
Transformed
Ligation pending/Failed
Awaiting GeneArt
Cloning difficulties
Part Number Part Name Status
K143001 5' AmyE Integration Sequence Sequenced
K143002 3' AmyE Integration Sequence Sequenced
K143005 5' EpsE Integration Sequence PCR Verified
K143006 3' EpsE Integration Sequence Sent for Sequencing
K143008 5' PyrD Integration Sequence Pending
K143009 3' PyrD Integration Sequence PCR verified
K143031 Aad9 Spectinomycin Resistance Gene Sent for Sequencing
K143032 EpsE Molecular Clutch Gene of B. subtilis Sequenced
K143033 LacI (Lva-, N terminal deletion) Sequenced
K143034 LipA-EAK16 Fusion Partially Sequenced
K143035 LipA-Human Elastin Fusion Transformed
K143036 Xylose Operon Regulatory Protein PCR verified
K143037 Ytva Blue Light Receptor of B. subtilis PCR verified
K143038 SacB-EAK16 Fusion Sequenced
K143039 SacB-Human Elastin Fusion Fails to clone
K143050 P43-gsiB Sent for Sequencing
K143051 P43-spoVG Sequenced
K143052 Pveg-gsiB Sequenced
K143053 Pveg-spoVG Sequenced
K143054 Phyperspank-gsiB Pending
K143055 Phyperspank-spoVG PCR verified
K143056 Pxyl-gsiB Pending
K143057 Pxyl-spoVG Pending
K143058 Pctc-gsiB PCR verified
K143059 Pctc-spoVG PCR verified
K143060 PgsiB-gsiB Wrong insert cloned
K143061 PgsiB-spoVG Sequenced
K143062 LacI - Terminator Pending
K143063 XylR - Terminator Pending
K143064 CAT - Terminator Sent for Sequencing
K143065 Aad9 - Terminator Pending

<html>
    <a href="#">Back to top</a>
</html>