User:Meng Xiao He/Notebook/fall08/2008/11/26: Difference between revisions
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===Colony PCR=== | ===Colony PCR=== | ||
* Platinum gradient PCR to test conditions for cbb and Duo | * Platinum gradient PCR to test conditions for cbb and Duo | ||
* Platinum PCR with 2 cco colonies at 57C | * Platinum PCR with 2 cco colonies at 57C and 1 direction determinant (relative to M13F sequencing primer) | ||
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Revision as of 12:55, 26 November 2008
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Double checking
UpdatesSpin down
2 colonies from each plate streaked onto new LB Gm plate to ensure they're not contaminants Suicide vector loss
Conjugation cco
Colony PCR
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