LB Media: Difference between revisions

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New page: *Weight out 25g of LB Broth powder *Transfer to a large beaker, then add 1000mL deionized water, mix *Split solution in half (two separate bottles) *(for making plates, add 7.5g of agar to...
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Revision as of 21:06, 28 April 2009

  • Weight out 25g of LB Broth powder
  • Transfer to a large beaker, then add 1000mL deionized water, mix
  • Split solution in half (two separate bottles)
  • (for making plates, add 7.5g of agar to one of the bottles and mix well)
  • autoclave both bottles (setting:45min. liquid)