IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/03: Difference between revisions
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** Plates were left on Roza's bench at 10:26 a.m. | ** Plates were left on Roza's bench at 10:26 a.m. | ||
==Preparation of LB-Agar Plates | ==Preparation of LB-Agar Plates== | ||
* In anticipation of the need for more LB-Agar, I will make more LB-Agar and ask someone to pour the plates this afternoon when it is autoclaved. | * In anticipation of the need for more LB-Agar, I will make more LB-Agar and ask someone to pour the plates this afternoon when it is autoclaved. | ||
* LB Agar composition was: | * LB Agar composition was: | ||
** 13g of LB-Agar in 350 mL of sdH2O. | ** 13g of LB-Agar in 350 mL of sdH2O. | ||
** Prepared two bottles. | ** Prepared two bottles. | ||
==Chloramphenicol Testing for Paul's new batch== | |||
* Paul brought over a new batch of chloramphenicol from his lab for us to use. | |||
* Stock was made at 25 mg/mL in 100% MeOH. | |||
* Steps taken to test chloramphenicol: | |||
** Spread plated 25 µL of new chloramphenicol onto 1 LB agar plate (Chlor 1). LB-only is the control (Chlor 0) | |||
** Let dry for 10 minutes. | |||
** Streaked DB3.1 and DH5a onto each of the plates as done previously. | |||
** Stored at Lagally Lab 37ºC incubator at 11:43 AM. | |||
** Paul's chloramphenicol stored at Lagally Lab -20ºC freezer; will make aliquots of them after confirmation of efficacy. | |||
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Revision as of 15:49, 15 June 2009
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Chloramphenicol Testing Results
Preparation of LB-Agar Plates
Chloramphenicol Testing for Paul's new batch
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