|
Strain or plasmid |
Relevant properties or genotype |
Source |
|
|
Strains |
|
|
|
E. coli DH5<IMG SRC="/giflibrary/12/alpha.gif" BORDER="0"> |
F-, <IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM">80d, lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(lacZYA-argF)U169 |
[a] |
|
E. coli M15 [pREP4] |
F-, lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi-1, lac-, mtl-, recA+ , KmR |
[a] |
|
Pseudomonas putida KT2440 |
type strain |
[b] |
|
Synechocystis sp. PCC 6803 |
type strain |
[c] |
|
Plasmids |
|
|
|
pCAR16 |
<IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, AmpR |
[<A HREF="#BIB27">27</A>] |
|
pJF119EH |
cloning vector, tac-promoter, IPTG inducible, AmpR |
[<A HREF="#BIB25">25</A>] |
|
pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N |
cloning vector, RBS, tac-promoter, IPTG inducible, AmpR |
this study |
|
pAW229 |
cloning vector, RBS, rha-promoter, L-rhamnose inducible, CmR |
[<A HREF="#BIB26">26</A>] |
|
pQE31-VTE1 |
tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, AmpR |
[<A HREF="#BIB18A">18a</A>] |
|
pCAS2JF |
1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH |
this study |
|
pCAS8 |
1.1 kb NdeI/BamHI PCR fragment ggh in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N |
this study |
|
pCAS11 |
0.9 kb BglII/BamHI PCR fragment crtE in pCAS8 |
this study |
|
pCAS12 |
1.0 kb BglII/BamHI PCR fragment hpt in pCAS11 |
this study |
|
pCAS15 |
1.2 kb NdeI/BamHI PCR fragment hpd in pAW229 |
this study |
|
pCAS18 |
1.2 kb BglII/BamHI PCR fragment hpd in pCAS11 |
this study |
|
pCAS19 |
1.0 kb NdeI/BamHI PCR fragment hpt in pAW229 |
this study |
|
pCAS23 |
1.0 kb NdeI/BamHI PCR fragment hpt in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N |
this study |
|
pCAS24 |
0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23 |
this study |
|
pCAS27 |
1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229 |
this study |
|
pCAS29 |
1.2 kb BglII/BamHI PCR fragment hpd in pCAS18 |
this study |
|
pCAS30 |
0.9 kb NdeI/BamHI PCR fragment crtE in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N |
this study |
|
pCAS37 |
1.2 kb BglII/BamHI PCR fragment hpd in pCAS24 |
this study |
|
pCAS47 |
1.3 kb BglII PCR fragment vte1 in pCAS29 |
this study |
