Yu:Northern solns: Difference between revisions
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Added Northern solution recipes |
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Revision as of 15:00, 10 September 2009
Northern Solutions
| Final Concentration | For 1 Liter | For 4 Liters |
| 3M NaCl | 175.3 g | 701.2 g |
| 0.3M NaCitrate | 88.2 g | 352.8 g |
| milliQ H2O | to 1 L | to 4 L |
| Final Concentration | For 500 mL | For 1 Liter |
| 0.4 M Morpholinopropanesulfonic (MOPS) acid (free acid) | 41.9 g MOPS | 83.8 g MOPS |
| 0.1 M Na-acetate-3 x H2O | 6.8 g | 13.6 g |
| 10 mM EDTA | 10 mL 0.5 M EDTA | 20 mL 0.5 M EDTA |
| Adjust pH to 7.2 with NaOH | ||
| Store in the Dark at 4 °C | ||
