Yu:TAE: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Michaelcyu (talk | contribs) 50xTAE |
mNo edit summary |
||
| Line 24: | Line 24: | ||
| | | | ||
|} | |} | ||
[[Category:Yu Solutions]] | |||
Latest revision as of 21:00, 22 September 2009
| 1. Measure 600mL H2O into a 2L plastic beaker. | 600mL H2O |
| 2. Weigh 242g Tris base and add to mixture | 242g Tris base |
| 3. Measure 57.1mL acetic acid (glacial) and add to mixture | 57.1mL acetic acid (glacial) |
| 4. Measure 100mL .5M EDTA and add to mixture | 100mL .5M EDTA |
| 5. Stir until completely dissolved | |
| 6. Bring volume to 1L in a graduated cylinder | Volume to 1L |
| 7. Dilute from 50x to 1x (1:50) for use. |
