IT 226/Fall 2009

Course overview

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Focuses on nucleic acid manipulation. Modules include, making a eukaryotic library, identifying clones, sub-cloning into a bacterial expression vector and verification of the clone's identity by restriction analysis and DNA sequencing. Basic laboratory techniques (solution making, buffer preparation, good safety techniques), sterile technique and compliance procedures.

The objective of this course is to introduce the student to the step-by-step process of scientific discovery while developing adept technique in the application of several basic procedures commonly used in biotechnology research in both academic and industrial settings. Students will maintain a scientific notebook, learn to apply experimental design, develop critical thinking skills in the critique of journal articles, and use computer databases. More specifically, this course focuses on current laboratory techniques used to isolate, manipulate and identify biological molecules such as nucleic acids and proteins. Basic laboratory techniques (solution making, buffer preparation, good safety techniques), sterile technique and compliance procedures will also be discussed.

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