Template:SBB10 ConstructionFiles BenB sbb01: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
New page: <pre> Construction of sbb01: N15 Protelomerase PCR BDBn15001F/BDBn15001R on Bca1455 (942 bp, gp=A) PCR BDBn15002F/BDBn15002R on Bca1485 (1020 bp, gp=B) ---------------------------------... |
(No difference)
|
Revision as of 20:35, 9 February 2010
Construction of sbb01: N15 Protelomerase
PCR BDBn15001F/BDBn15001R on Bca1455 (942 bp, gp=A)
PCR BDBn15002F/BDBn15002R on Bca1485 (1020 bp, gp=B)
--------------------------------------------------------------
PCR BDBn15001F/BDBn15002R on A+B (1924 bp, EcoR1/BamH1)
Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb01 {rbs.part!}
BDBn15001F Forward retrieval, RBS introduction and BioBricking on Bca1455
CCATAgaattcATGagatctTCAATCTCGGAGGAACGGTAAGGAAGGAAAACatgAGCAAGGTAAAAATCGGTGAG
BDBn15001R Reverse retrieval and SOE-site introduciton on Bca1455
CGCTTTTATCTTCACTGCGTTTTTTAGCTTGCCCTGAG
BDBn15002F Forward retrieval and SOE-site introduciton on Bca1485
CTCAGGGCAAGCTAAAAAACGCAGTGAAGATAAAAGCG
BDBn15002R Reverse retrieval and BioBricking on Bca1485
CTGATggatccGCTGTAGTACGTTTCCCATGCGG
