IGEM:MIT/2006/Notebook/2006-6-8

SAMT from A. Majus

1. Forward Strand
   • Sequence to order: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG -3'
   • Length: 51 bp
   • GC content: 43.1%
   • Melt Temp. 67.0 C

1. Reverse Strand
   • Sequence to order: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAG TTT CTT TTT GCT TG -3'
   • Length: 50 bp
   • GC content: 42.0%
   • Melt Temp. 65.9 C

BAMT from A. majus

• Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG AAA GTG ATG AAG AAA C
  • Length: 49
  • GC Content:
  • Melting Temp:

• Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AG
  • Length: 49
  • GC Content: 44.9%
  • Melting Temp: 66.7

BSMT (1 and 2) from P. x hybrida

Designed Forward Strand: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT -3'

• LENGTH: 48
  
• GC CONTENT: 45.8 %
  
• MELT TEMP: 67.7 ºC
  

Designed Reverse Strand: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AA -3'

• LENGTH: 50
  
• GC CONTENT: 38.0 %
  
• MELT TEMP: 64.9 ºC
  

ATF1 from S. Cerevisiae

Designed forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG AAT GAA ATC GAT GAG -3'

Length: 48 bp GC content: 45.8% Melt temp: 66.7 deg. C

Designed reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA GGG CCT AAA AGG AGA G -3' Length: 52 bp GC content: 48.1% Melt temp: 67.9 deg. C