Holmesbr/plan: Difference between revisions
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==RNAi Knockdown of RNase H1 <i>in situ</i>== | ==RNAi Knockdown of RNase H1 <i>in situ</i>== | ||
===miRNA=== | |||
I have 4 miRNA constructs plus a negative control miRNA construct. I have tested these against endogenous RNase H1 in 3T3 cells, but the knock-down by RT-RT-qPCR has been abysmal, with only one construct acheiving ~40% knock-down. However, this may be due to poor Transfection Efficiency | |||
===Transfection Efficiency=== | |||
==Immunolabeling RNase H1, Top3a and POLG== | ==Immunolabeling RNase H1, Top3a and POLG== | ||
Revision as of 19:53, 14 June 2006
Introduction
This page is meant to keep my two mentors up-to-date on what I am working on and also help keep myself organized.
Specific Aims
RNAi Knockdown of RNase H1 in situ
miRNA
I have 4 miRNA constructs plus a negative control miRNA construct. I have tested these against endogenous RNase H1 in 3T3 cells, but the knock-down by RT-RT-qPCR has been abysmal, with only one construct acheiving ~40% knock-down. However, this may be due to poor Transfection Efficiency
