IGEM:IMPERIAL/2006/project/parts/BBa J37015/Cre-LoxP system

• Cre recombinase: The universal reagent for genome tailoring
• Cre recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein
• Using cre-recombinase as a switch appears to be, in theory, a very efficient and solid method.
• The parts are not available in the registry however, which means that we would have to make them.
• The loxP sites are around 20 bases in length and so could be ordered fairly easily.
• The cre recombinase site is around a kilobase in length. However there is a good chance that we may be able to obtain this from one of the labs, and if not, plasmids containing the part are commercially available for us to order.
• The cre recombinase will also need a promoter that we can activate (eg lacI)
• If the parts can be obtained, then we estimate that the length of time for implementation should be under a week.
• Proposed Implementation of Cre-LoxP system