User:Derek J. Sanchez/Notebook/TB Sensor - MS applied project/2012/03/08: Difference between revisions
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Find the size of expected part and plasmid back bone and compare to chemical ruler. <br> | Find the size of expected part and plasmid back bone and compare to chemical ruler. <br> | ||
<br> | <br> | ||
Gel was removed form case and placed on UV viewing stand, and a picture was taken. | |||
==Results (Will be edited with my results by no later than 3/9/2012, current info is property of Dr. Haynes.== | |||
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'''Minipreps'''<br> | |||
> Check KAH213-218 with E/P<br> | |||
> Check KAH207-209/V0200 with N/S | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> | |||
| rowspan="7" | <u>Expected:</u><br>V0120 = ~3200<br>1,2. KAH213 = 2834<br>3,4. KAH214 = 1757<br>5,6. KAH215 = 2057<br>7,8. KAH216 = 2590<br>9,10. KAH217 = 1513<br>11,12. KAH218 = 1813<br><br><br>V0200 = 4442<br>13,14. KAH207/V0200 = 1836<br>15,16. KAH208/V0200 = 1884<br>17,18. KAH209/V0200 = 1932 | |||
| rowspan="7" | [[Image:KAH_071311_gel1.tif|300px|Miniprep digests]]<br>15 μL/lane; 1% agarose<br><br>[[Image:KAH_071311_gel2.tif|160px|Miniprep digests]]<br>15 μL/lane; 1% agarose | |||
|- | |||
| DNA(plasmid) || 2.0 μL | |||
|- | |||
| 10X buffer || 1.5 | |||
|- | |||
| EcoRI || 1.0 | |||
|- | |||
| PstI || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 9.5 | |||
|- | |||
| || 15 μL --> 37°C/ ~15 min. | |||
|} | |||
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Revision as of 13:24, 8 March 2012
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03/08/12Checking the size of created DNAThe DNA samples prepared yesterday today will be tested for size with electrophoreses.
Results (Will be edited with my results by no later than 3/9/2012, current info is property of Dr. Haynes.Minipreps |