BME494 Project Group2: Difference between revisions
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==ABSTRACT== | ==ABSTRACT== | ||
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[[Image:Mussel.jpg|thumb|frame|left| | [[Image:Mussel.jpg|thumb|frame|left|A mussel exhibiting adhesion to a surface]] | ||
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Revision as of 14:15, 13 March 2012
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ABSTRACTWithin the foot of the mussel there are five mussel proteins that vary by amount. This varying amount is caused by the differing amounts of DOPA present within the proteins. These proteins are what cause the adhesive properties present within a mussel. What we aimed to do is create a controllable configuration of cells so that adhesion to a surface can be easily toggled. By using a combination of the existing natural part Mussel Adhesive Protein Mgfp-1 and the pre-existing part Mgfp-5 (that was bio-bricked and used by UC Berkeley) a unique bioadhesive that provides desirable adhesion properties can be created.
BACKGROUNDThe inspiration for this project came from the blue mussel's (Mytilus edulis) ability to stick to surfaces. In order to attach to a surface they spin a set of threads from their stem that are able to attach to the surface via plaques. These plaques then connect the substrate and byssal thread. The strength of the adhesion in then determined by the amount of threads that are employed. Bioadhesives are natural polymeric materials that many organisms, such as blue mussels aforementioned, used for their adhesive properties. Bioadhesives have been seen as an excellent alternative to human-made substances as they are very and durable in conjunction with being eco-friendly. In addition, they exhibit properties that allow them to be used in underwater and medical applications which is the main interest of this project.
PROOF OF CONCEPT DESIGNNew Natural Part
The end of the Mefp-1 protein sequence:TATCCATCACAATATTAA
Key Pre-existing Part
TESTINGMeasurement
We expect to see a quasi-linear relationship between adhesive power and glowing intensity. This is because the GFP is being produced in the same plasmid cycle as the adhesive protein. The adhesive power could be monitored to support these conclusion through high cost equipment such as the AFM cantilever.
HUMAN PRACTICESPETA Problems
If the bacteria somehow multiplied outside the intended region of interest it may be possible to accidently adhere things together
OUR TEAM
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