Talk:Knight:Purification of His-tagged proteins/Denaturing: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(No difference)
|
Revision as of 11:04, 21 August 2006
Sample results
Lane order:
- SeeBlue Plus2 Pre-Stained Standard
- Cleared lysate
- Flow through (after column binding)
- Flow through (after column binding, second time)
- Wash 1
- Wash 2
- Eluted protein
- Cleared lysate from cells not expressing a His-tagged protein
- Flow through (after column binding, second time) from cells not expressing a His-tagged protein
- Wash 2 from cells not expressing a His-tagged protein