Cytoplasm and nuclear protein extraction: Difference between revisions
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Harvest cells and wash with 1XPBS | Harvest cells and wash with 1XPBS | ||
#'''Cytoplasm extraction''' | =='''Cytoplasm extraction'''== | ||
#'''Cytoplasm extraction buffer''' | |||
#*10mM Hepes or Tris (pH7.5) | #*10mM Hepes or Tris (pH7.5) | ||
#*40mM KCL | #*40mM KCL | ||
Revision as of 19:18, 21 August 2006
Harvest cells and wash with 1XPBS
Cytoplasm extraction
- Cytoplasm extraction buffer
- 10mM Hepes or Tris (pH7.5)
- 40mM KCL
- 2mMMgCl2
- 10% glycerol
- 1mM NaPPi
- 1ug/mL pepstatin
- 1ug/mL aprotinin
- 1ug/mL leupeptin
- 1mM NaVO4
- 1mM NaF
- 1mM PMSF
- water to 50 mL
