Elizabeth Polidan Week11

Elizabeth Polidan

BIOL 398.03 / MATH 388

• Loyola Marymount University

• Los Angeles, CA, USA

• Email me through OpenWetWare

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Vocabulary

1. trehalose
2. mannoproteins
3. prototrophic
4. fatty-acid desaturase
5. cis-regulatory motifs
6. HXT5
7. HXT6

Outline of Tai et al. (2007)

1. Introduction
   1. What is the main result presented in this paper?
   2. What is the importance or significance of this work?
2. Methods
   1. How did they treat the cells (what experiment were they doing?)
   2. What strain(s) of yeast did they use? Was the strain haploid or diploid?
   3. What media did they grow them in? Under what conditions and temperatures?
   4. What controls did they use?
   5. How many replicates did they perform per condition?
3. Analysis
   1. What mathematical/statistical method did they use to analyze the data?
4. Discussion
   1. What transcription factors did they talk about?
   2. Briefly state the result shown in each of the figures and tables.

Placeholder for reflection questions.

Critically evaluate the Tai et al. (2007) paper.

1. Overall, do you think this paper was clearly written? Why or why not?
2. Based on what is written in the methods section, do you think you could reproduce their experiments and data analysis?
3. What else would you like to know about their methods, results, and future directions?