BME494s2013 Project Team2

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Overview & Purpose Text describing the image Background Wow the Lac Operon The Lac Operon is a gene specific to E. Coli that controls the cell's digestion of lactose. It consists of a promoter, an operator, three structural genes, and a terminator. It is both positively and negatively regulated, allowing expression to be contingent on the concentrations of different sugars in the cell. Expression of the Lac Operon is determined jointly by the levels of Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Insert crap about the Lac Operon. Wow the Lac Operon Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Keep talking about the Lac Operon. Design: Our genetic circuit OUR GENE SWITCH: Device design. Image adapted from Building: Assembly Scheme Testing: Modeling and GFP Imaging A LAC SWITCH MODEL We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated. AN INTERACTIVE MODEL We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system. COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions. Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model. Human Practices Danger of Chemicals in Farmlands Our Team Shay Ravacchioli My name is Shay Ravacchioli, and I am a Junior majoring in Biomedical Engineering with minors in Biological Sciences and Psychology. I am taking BME 494 because I think Synthetic Biology is fascinating. An interesting fact about me is that I play piano and guitar. Jenessa Lancaster My name is Jenessa Lancaster, and I am a Junior majoring in Biomedical Engineering with a minor in Psychology. I am taking BME 494 because I have always wanted to learn more about Synthetic Biology and Genetic Engineering. An interesting fact about me is that I write songs. Michael Rose My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###. Your Name My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###. Works Cited [1] Heller, H. Craig., David M. Hillis, Gordon H. Orians, William K. Purves, and David Sadava. Life: The Science of Biology. Sunderland, MA,: Sinauer Ass., W.H. Freeman and, 2008. N. pag. Print. [2] Full reference. [3] Full reference.