Biomod/2013/Aarhus/Supplementary/Supplementary Data: Difference between revisions

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===Cholesterol===
===Cholesterol===
[[Image:Biomod_Aarhus_Chem_NMR_4H.png|800x800px|Center]]
===Photosensitizer===
===Photosensitizer===
===Peptide lock===
===Peptide lock===

Revision as of 14:01, 24 October 2013

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Supplementary data

Origami design

Details of the different modules of the plate.
Details of the dome structure.
Details of the different modules.

sisiRNA

Yields of RP-HPLC purifications of the modified strands

Table sisi 1: Yields of RP-HPLC purifications of the 12 modified strands.

Estimated yields of the annealing reactions

Estimated yields of the annealing reactions.

Luciferase knockdown with all singly modified duplexes

Cell viability corrected luciferase activities for all singly modified duplexes, transfected with Lipofectamine. Click on the image and choose 'high resolution' for details.

Best fit IC50 values of all knockdown experiments using singly modified duplexes using Lipofectamine.The IC50 value for W179-PEG1K was calculated with a low R2 value and is not conclusive.
Luciferase and MTT measuremetns of all knockdown experiments with singly modified duplexes using Lipofectamine.

System in action

Radioactive labeling of W376

Fig. 1) 25 bp DNA ladder, 2) 6 µL labeled W376, 3) 1 pmol W376, 4) 1.5 pmol W376, 5) pmol W376, 6) 2.5 pmol W376, 7) pmol W376.

The band containing the labeled sample appears most similar to that in lane 4 which means that the 6 µL that were run on the gel contained 1.5 pmol W376, corresponding to a concentration of 0.25 µM in the purified sample.

Cholesterol

Center

Photosensitizer

Peptide lock

Spectra

(S)-2-((tert-butoxycarbonyl)amino)-5-methoxy-5-oxopentanoic acid

1H NMR File:Exp4HNMRfinishedforrealz-eps-converted-to.pdf

13C NMR File:Exp4CNMRfinishedforrealz-eps-converted-to.pdf

ddUTP

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SITEMAP | BIOMOD 2013 NANO CREATORS | Aarhus University

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