User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/31: Difference between revisions
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| Incubate at room temperature for 30 minutes. | | Incubate at room temperature for 30 minutes. | ||
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| '''Transformation result''' || | | '''Transformation result''' || || || || <span style="color:#FF0000">---</span>|| <span style="color:#FF0000">---</span> | ||
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* Fast transformation protocol in DH5α-T. Add 30 μL of cells to each sample and incubate on ice for 30 minutes. | * Fast transformation protocol in DH5α-T. Add 30 μL of cells to each sample and incubate on ice for 30 minutes. | ||
* Grow on AMP agar plates and incubate overnight at 37°C. | * Grow on AMP agar plates and incubate overnight at 37°C. |
Revision as of 17:19, 31 October 2013
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