User:Helen L. Slucher/Notebook/CHEM-572/2014/02/11: Difference between revisions
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* Test the activity of citrate-HRP-GNPs using O2 binding assays | * Test the activity of citrate-HRP-GNPs using O2 binding assays | ||
==Procedure== | |||
Activity of citrate-HRP-GNPs | |||
* We are going to use an assay provided by Dr. Hartings | |||
**The assay uses a reagent that changes color when HRP is added in addition to hydrogen peroxide | |||
**The hydrogen peroxide alone will change the color of the solution, so we will be comparing hydrogen peroxide alone with hydrogen peroxide and GNPs | |||
*We also are going to run UV Vis of the samples to see how the absorption spectra changes over time | |||
Dopamine Coating | |||
#Dissolve dopamine in 100mL Tris Buffer to have a 10mM solution at pH=8.5 | |||
#Mix 17mL of GNP solution with 17mL of the dopamine solution | |||
#Stir under air at room temperature for 1 hour | |||
#Purify by centrifuging and then redisperse in water. | |||
#Filtrate through syringe filters with cellulose acetate membranes with pore size of 1um | |||
#Make 2 solutions. | |||
## to be stored over night on lab bench | |||
## to be stored over night in the refrigerator | |||
==Notes== | |||
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Revision as of 10:31, 11 February 2014
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Objective
ProcedureActivity of citrate-HRP-GNPs
Dopamine Coating
Notes |