WRB100806-Construction of pSB1AK3-J23120: Difference between revisions

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[OriT]-[pTet][Lock3][RFP][TT] <br>
[OriT]-[pTet][Lock3d w/ 5' UTR ext][RFP][TT] <br>
digest pSB1AK3-J23039 (EcoR1, Spe) <br>  
digest pSB1AK3-J23039 (EcoR1/PstI, EcoRI buf, large 3152bp) <br>  
digest pSB1C3-J23077 (EcoR1, Spe) <br>
digest pSB3C6-J23077 (XbaI/PstI, neb3, small 985bp) <br>
digest pSB1A2-J01003 (Xba, Pst) <br>
digest pSB1A2-J01003 (EcoRI/SpeI, EcoRI buf, small 394bp) <br>
ligate, product is pSB1AK3-J23120 <br>
ligate, product is pSB1AK3-J23120 <br>

Latest revision as of 20:47, 11 October 2006

[OriT]-[pTet][Lock3d w/ 5' UTR ext][RFP][TT]
digest pSB1AK3-J23039 (EcoR1/PstI, EcoRI buf, large 3152bp)
digest pSB3C6-J23077 (XbaI/PstI, neb3, small 985bp)
digest pSB1A2-J01003 (EcoRI/SpeI, EcoRI buf, small 394bp)
ligate, product is pSB1AK3-J23120

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