Biomod/2014/fit Results and Discussion.html: Difference between revisions
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<td><img alt="" src="/images/1/16/FitsilicaT.png" width="250" height="250" border="0" /></td> | <td><div style="width:260px;"><img alt="" src="/images/1/16/FitsilicaT.png" width="250" height="250" border="0" /><p>(*^_^*)</p></div></td> | ||
<td><img alt="" src="/images/4/48/FitsilicaF.png" width="250" height="250" border="0" /></td> | <td><div style="width:260px;"><img alt="" src="/images/4/48/FitsilicaF.png" width="250" height="250" border="0" /><p>(*^_^*)</p></div></td> | ||
<td><img alt="" src="/images/0/09/FitsilicaTF.png" width="250" height="250" border="0" /><td> </table> | <td><div style="width:260px;"><img alt="" src="/images/0/09/FitsilicaTF.png" width="250" height="250" border="0" /><p>(*^_^*)</p></div><td> </table> | ||
figure 3 shows the confocal microscope image of mixture of TAMURA labeled DNA bind silica particles and unlabeled silica particles. Fluoroscent of TAMURA was observed around the silica particles. We confirmed that TAMURA labeled DNA bind silica particles were synthesis as expected. | figure 3 shows the confocal microscope image of mixture of TAMURA labeled DNA bind silica particles and unlabeled silica particles. Fluoroscent of TAMURA was observed around the silica particles. We confirmed that TAMURA labeled DNA bind silica particles were synthesis as expected. | ||
Revision as of 17:22, 29 August 2014
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Team FIT
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| <a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka">Top</a> | <a href="fit_Introduction.html">Introduction</a> | <a href="fit_Approach and Goals.html">Approach and Goals</a> | <a href="fit_Method.html">Method</a> | <a href="fit_Results and Discussion.html">Results and Discussion</a> | <a href="fit_Member.html">Member</a> | <a href="fit_Sponsor.html">Sponsor</a> |
Results and Discussion
<img alt="" src="/images/b/b8/Fitkyosilica.png" width="300" height="225" border="0"/ align="left" style="margin-right: 10px;">
左図は合成したメソポーラスシリカを観察したものである。シリカの粒径は300から1000nmと不均一であり、メソポーラスの大きさもばらばらであった。目の粗さが1μmのメンブレーメンフィルターを用いて粒径を均等にしようと試みたが、ばらつきがでてしまった。原因を考えるとともに、今よりも細かい目のフィルターで吸引濾過をする。またばらつきによって結果の誤差が生じるかなど比較実験を行う。
| <img alt="" src="/images/4/4c/Fitkspec.png" width="350" height="350" border="0" /> | <img alt="" src="/images/0/0b/Fitfisilica.png" width="350" height="350" border="0" /> |
Figure 2 shows the fluorescent spectral 100μM FITC labeled DNA, 100μM TAMRA labeled DNA and mixture of two DNAs. Fluorescent of FITC-labeled DNA and TAMra labeled were 520 and 580nm at excitation of 480nm light, respectively. Only 580nm fluorescent was observed from the mixture of two DNAs. 520nm fluorescent from FITC was used for the excitation of TAMRA, because two DNAs formed double strand DNA and FITC were located head TAMRA.
figure 1 shows the fluorescent spectra of 50pM Fruoroscene, 50pM TAMURA labeled DNA and mixture of Flioroscene and TAMURA labeled DNA. Fluorescent of fluorescene and TAMURA were 520 and 580nm at excitation of 488nm light, respectively. In this experiment, since there was no FRET effect mixture spectra was consisted with addition of two spectrum.
<img alt="" src="/images/1/16/FitsilicaT.png" width="250" height="250" border="0" /> (*^_^*) |
<img alt="" src="/images/4/48/FitsilicaF.png" width="250" height="250" border="0" /> (*^_^*) |
<img alt="" src="/images/0/09/FitsilicaTF.png" width="250" height="250" border="0" /> (*^_^*) |
figure 3 shows the confocal microscope image of mixture of TAMURA labeled DNA bind silica particles and unlabeled silica particles. Fluoroscent of TAMURA was observed around the silica particles. We confirmed that TAMURA labeled DNA bind silica particles were synthesis as expected.
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