Penn State University 2006:lbplates: Difference between revisions
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=LB Plates= | |||
(per/L H2O) | |||
===<font color="green">Procedure</font>=== | |||
1. Weigh and add to H2O in large Erlenmeyer: | 1. Weigh and add to H2O in large Erlenmeyer: | ||
*10 g Bacto-tryptone | |||
*5 g Bacto-yeast extract | |||
*10 g NaCl | |||
*15 g Bacto-agar | |||
2. Autoclave on slow exhaust cycle 25 min | |||
3. Cool in tap-water-bath 10 min, then place in 45ºC water bath. | |||
3. Cool in tap-water-bath 10 min, then place in 45ºC water bath. | |||
(in sterile hood) | (in sterile hood) | ||
4. When media has cooled to ~45ºC (comfortable to hold), add appropriate antibiotic. | |||
5. Pour plates (~25 mL/plate) | 4. When media has cooled to ~45ºC (comfortable to hold), add appropriate antibiotic. | ||
6. Allow to solidify & store in bag in cold-room | |||
5. Pour plates (~25 mL/plate) | |||
6. Allow to solidify & store in bag in cold-room | |||
'' | '' | ||
[http://openwetware.org/wiki/IGEM:PennState Main] | [http://openwetware.org/wiki/IGEM:PennState Main] | ||
Latest revision as of 00:04, 25 October 2006
LB Plates
(per/L H2O)
Procedure
1. Weigh and add to H2O in large Erlenmeyer:
- 10 g Bacto-tryptone
- 5 g Bacto-yeast extract
- 10 g NaCl
- 15 g Bacto-agar
2. Autoclave on slow exhaust cycle 25 min
3. Cool in tap-water-bath 10 min, then place in 45ºC water bath. (in sterile hood)
4. When media has cooled to ~45ºC (comfortable to hold), add appropriate antibiotic.
5. Pour plates (~25 mL/plate)
6. Allow to solidify & store in bag in cold-room
