Lidstrom:Sorvall Centrifuge: Difference between revisions
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== Instructions == | == Instructions == | ||
# pre-chill the rotor you plan to use (overnight in fridge) | |||
# turn on centrifuge | |||
# | |||
== Sample protocols == | |||
=== Example protocol for SIP3-4: === | |||
Janet's SIP3-4 centrifugation: | Janet's SIP3-4 centrifugation: | ||
* Chill to 4<sup>o</sup>C with SS-34 rotor inside | * Chill to 4<sup>o</sup>C with SS-34 rotor inside | ||
* Fill correct tubes | * Fill correct tubes | ||
* Spin for ___ min at ___ RCF at ___<sup>o</sup>C | * Spin for ___ min at ___ RCF at ___<sup>o</sup>C | ||
=== Example protocol for AM1: === | |||
Ask Nate/Ceci | |||
== Centrifugation Math & Basics == | == Centrifugation Math & Basics == |
Revision as of 10:10, 26 January 2015
Back to Protocols
Background
We have a Sorvoll RC-5B centrifuge that is useful for spinning large volumes in 0.5L bottles or medium-sized (a few mL) volumes very quickly
Rotors
SS-34
- Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- BUT Katya (circa 2010) recommends using it at <= 18,000 RPM
- Tubes:
- Can't be autoclaved.
- Actually, Amanda might have autoclaved them before.
- Can't write on body; can only write on cap. Sandy says stickers don't stick.
- Can't be autoclaved.
GS-3
- GS-3.
- BUT Katya (circa 2010) recommends using it at <= 7,500
- For bigger bottles. (0.5L)
- Holds eppendorf (1.5mL) tubes
- Spins at 9,000 RPM/13,700 RCF
Explanation of RCF
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
- There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.
Instructions
- pre-chill the rotor you plan to use (overnight in fridge)
- turn on centrifuge
Sample protocols
Example protocol for SIP3-4:
Janet's SIP3-4 centrifugation:
- Chill to 4oC with SS-34 rotor inside
- Fill correct tubes
- Spin for ___ min at ___ RCF at ___oC
Example protocol for AM1:
Ask Nate/Ceci
Centrifugation Math & Basics
- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).