User:Torsten Waldminghaus/qPCR-Primers

Name	Sequence	Characteristics
uvrDfw	AGTTCCCGCAGGTGTTTATC	qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from [1])
uvrDrv	GTCAGCGTCAGTTTCTGCAT	qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from [1])
uvrDprobe	AGACGCCCGCCTTCATCCAG	HPLC-pure 5'Fam - 3'Tamra qPCR for uvrD-region on E. coli chromosome with no GATC-sites (Region B from [1]
yahEFfw	CCATCGAGACGATCAAAGAA	qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])
yahEFrv	CAGCATCTGGCTTTGTTGTT	qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])
yahEFprobe	AACTCGCGTCCTTCGGCAGC	HPLC5'Fam - 3'Tamra qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])
cluster-fw	CTGACTGATGAGATCCAACGA	qPCR for GATC-cluster	cluster-rv	CTGGTGCTACGCCTGAATAA	qPCR for GATC-cluster	cluster-p	AAATTCGACCCGGCTGTCGC	qPCR for GATC-cluster

1. Yamazoe M, Adachi S, Kanaya S, Ohsumi K, and Hiraga S. Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli. Mol Microbiol. 2005 Jan;55(1):289-98. DOI:10.1111/j.1365-2958.2004.04389.x |