Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis

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Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.



T/R lab

Recovery gel

Here is your recovery gel: Error creating thumbnail: File missing

Top Gel (numbers refer to lanes):

  • 1-2 Pink
  • 3-4 Purple
  • 5 MW
  • 6-7 White
  • 8-9 Blue

Bottom Gel (numbers refer to lanes):

  • 1-2 Orange
  • 3-4 Yellow
  • 5 MW
  • 6-7 Green
  • 8-9 Platinum
  • 10 Red

Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).

Pre-purification gels (for reference/posterity)

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!

Error creating thumbnail: File missing
Red Group
Error creating thumbnail: File missing
Orange Group
Error creating thumbnail: File missing
Yellow Group
Error creating thumbnail: File missing
Green Group
Error creating thumbnail: File with dimensions greater than 12.5 MP
Blue Group
Error creating thumbnail: File missing
Pink Group
Error creating thumbnail: File missing
Purple Group
Error creating thumbnail: File missing
Silver Group
Error creating thumbnail: File missing
White Group


W/F lab

Recovery gel

Error creating thumbnail: File missing
W/F Recovery Gel

Pre-purification gels (for reference/posterity)

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!

Error creating thumbnail: File missing
Red Group
Error creating thumbnail: File missing
Orange Group
Error creating thumbnail: File missing
Yellow Group
Error creating thumbnail: File missing
Green Group
Error creating thumbnail: File missing
Blue Group
Error creating thumbnail: File missing
Pink Group
Error creating thumbnail: File missing
Purple Group
Error creating thumbnail: File missing
White Group