Matt Gethers

I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.

20.109 Work

M13 Engineering Ideas

Extractions, separations, etc. include ensuring that each gene has its own promoter, RBS, terminator, and any other pertinent regulatory sequences. In addition to separating the genes, it would also be wise to develop or utilize/modify an existing system like Biobricks to build the genome so that convenient restriction sites exist between the genes so they can be easily removed or replaced.

Gene	Modification
X	Extract from gene II
II	Extract gene X
V	Add some base pairs between V and VII to allow for a restriction site
VII	Separate from gene IX
III	Change the GTG to ATG Start?
VI
I	Separate from genes IV and XI
XI	Separate from genes I and IV
IV	Separate from genes I and XI
M13 ORI 1 & 2	Are two ORIs necessary? If so, can they be consolidated?
KanR