BioMicroCenter:Protocols

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Revision as of 21:35, 22 April 2009 by Allison Perrotta (talk | contribs) (→ILLUMINA SEQUENCING PROTOCOLS)
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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- DATA MANAGEMENT -- OTHER TECHNOLOGY

BioMicro Center Protocol Testing

  • RT-PCR Protocol for Sample QC
  • Cluster Visualization Protocol for Flowcell QC

ILLUMINA SEQUENCING PROTOCOLS

Cluster Station and Genome Analyzer

  • Priming and Single Rread Protocol
  • Priming and Paired-End Protocol

ChIP Seq

  • Illumina Protocol

Digital Gene Expression

  • Illumina Protocol (NlaIII, full kit protocol)
  • Illumina Protocol (NlaIII, oligos only protocol)- unlike the full kit, this kit provides only the adapters PCR primers. Users wishing to adapting current lab protocols may find this protocol more useful.
  • Illumina Protocol (DpnII, full kit protocol)
  • Illumina Protocol (DpnII, olgios only protocol)


mRNA Seq

  • Illumina Protocol


Small RNA Seq

  • Tuschl Lab Protocol
  • Illumina Protocol (full kit protocol)
  • Illumina Protocol (oligos only protocol)
  • Illumina Protocol (Alternate v1.5 protocol)

Genomic DNA

  • Generic Illumina protocol
  • Illumina protocol (oligos only protocol)
  • Paired End Illumina Protocol
  • Paired End Multiplexing Illumina Protocol
  • Mate Pair Illumina Protocol (2-5kb samples)
Retrieved from "https://openwetware.org/mediawiki/index.php?title=BioMicroCenter:Protocols&oldid=303894"

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  • This page was last edited on 22 April 2009, at 21:35.
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