GFP

• wtGFP on NCBI

GFP Variants

• GFPmut3b - S65G, S72A
• GFPmut3* - mutations from wt: S2R,S65G,S72A
  • This is GFPmut3b[1] with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.[2]

References

1. Cormack BP, Valdivia RH, and Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 1996;173(1 Spec No):33-8. DOI:10.1016/0378-1119(95)00685-0 |
3. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, and Molin S. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol. 1998 Jun;64(6):2240-6. DOI:10.1128/AEM.64.6.2240-2246.1998 |

Molecular Biology and Muation of Green Fluorescent Protein (Book Chapter, Zacharias & Tsien)