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PCR Colony PCR Verification Enzymatic Inverse PCR (EI-PCR) Mega-Primer

Need-to-know Techniques General Restriction Digest DNA Ligation Beta-galactosidase Assay Tecan Safire2 E. coli/S. typhimurium specific DNA Transformation Transduction One-step Gene Distruption CRIM Plasmid Integration B. subtilis specific B. subtilis transformation

Media Preparation LB SOB/SOC M9/M9c Tryptone Broth (TB) Penassay Broth Motility Plates If your interested in more recipes go here

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