Stephanopoulos:Projects
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Research
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Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components: a) the modification of biochemical pathways inside cells and b) the rigorous evaluation of the resulting cellular phenotypes. In recent years our group has investigated the following topics:
- <a href="Coryne.shtml">Aminoacid biosynthesis in Corynebacterium glutamicum.</a>
- <a href="Rhodo.shtml">Indene bioconversion in Rhodococcus Sp.</a>
- <a href="Synecho.shtml">CO2 fixation and product synthesis by the cyanobacterium Synechocystis Sp.</a>
- <a href="Diabetes.shtml">Elucidating type II diabetes by linking the expression and metabolic phenotypes of hepatoma and hepatocyte cells.</a>
To accomplish the above goals we make use of a diverse array of scientific tools and
methods, many of which have also become areas of research for our group:
- <a href="Bioinfo.shtml">Bioinformatics and Systems Biology</a> - Our group was one of the first to realize the importance of computational tools for handling the large volume of data generated by microarrays and other technologies.
- <a href="Fluxes.shtml">Methods for intracellular flux determination</a> - Fluxes are determined by material balancing, NMR fine spectra analysis and GC-MS measurements.
- <a href="Microarray.shtml">DNA microarrays</a> - We have developed full genome microarrays for Synechocystis Sp., and partial microarrays for C. glutamicum, E. coli, and the mouse genomes.
- <a href="BioReactor.shtml">Bioreaction network analysis.</a> </body> </html>
